中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (26): 4208-4214.doi: 10.3969/j.issn.2095-4344.0933

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

铒铬铱钪镓石榴石激光照射乳牙牙本质粘接强度的变化

梁 韵1，史剑杰2，陈 柯3，罗震山1，邓文鑫1   

  1. 1广州医科大学附属第三医院荔湾医院，广东省广州市 510175；2广州医科大学附属第三医院，广东省广州市 510150；3广州市妇女儿童医疗中心，广东省广州市 510623
  • 收稿日期:2018-02-09
  • 通讯作者: 史剑杰，主任医师，广州医科大学附属第三医院口腔科，广东省广州市 510150
  • 作者简介:梁韵，女，1990年生，广东省肇庆市人，汉族，2017年暨南大学毕业，硕士，医师，主要从事口腔医学研究。
  • 基金资助:

    广东省自然科学基金项目(S2013010014879)；广州市科技计划项目(201510010160)

Irradiation with erbium, chromium:yttrium scandium gallium garnet laser alters shear bond strength to primary tooth dentin

Liang Yun1, Shi Jian-jie2, Chen Ke3, Luo Zhen-shan1, Deng Wen-xin1   

  1. 1Liwan Hospital, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510175, Guangdong Province, China; 2the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China; 3Guangzhou Women and Children’s Medical Center, Guangzhou 510623, Guangdong Province, China
  • Received:2018-02-09
  • Contact: Shi Jian-jie, Chief physician, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China
  • About author:Liang Yun, Master, Physician, Liwan Hospital, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510175, Guangdong Province, China
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. S2013010014879; the Science and Technology Research Plan of Guangzhou, No. 201510010160

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摘要：

文章快速阅读：

 

文题释义：

铒铬铱钪镓石榴石激光：是由铒铬铱钪镓石榴石晶体释放出的中红外激光，波长为2.78 μm，输出功率1-  6 W，脉宽140-200 μs，经光导纤维传导，其通过水动力学系统赋予水分子激光的能量而工作，照射过程中能量通过光纤传送到工作机头，水分子携带能量后具有超强动能，成为高速喷射的雾化水颗粒作用于光照处组织，引起“微爆破”，从而有效切割和消融牙体硬组织。

粘接强度：指引起粘接剂与被粘物体界面内或其邻近的粘接破坏时，单位粘接面积所能承受的最大毁坏力，为测量各种牙体表面处理方式对牙本质表面粘接力影响的主要指标。充填体界面的压力类型复杂，但可主要被看作是拉伸或剪切类型的压力，目前评价牙体粘接强度的实验技术主要包括拉伸粘接强度测试和剪切粘接强度测试。

 

摘要

背景：牙体硬组织表面预处理对粘接修复的成功起着重要作用。

目的：探讨铒铬铱钪镓石榴石激光酸蚀乳牙本质表面对树脂粘接牙本质剪切强度的影响。

方法：获取120颗离体人乳切牙，去除牙釉质后随机分成3组进行表面预处理：空白对照组(n=12)不进行任何处理；磷酸酸蚀组(n=12)采用35%磷酸酸蚀牙面；激光组(n=48)采用铒铬铱钪镓石榴石激光照射牙面，又分4个亚组，分别为1，2，3，4 W功率组(1 W、50 mJ、20 Hz；2 W、100 mJ、20 Hz；3 W、150 mJ、20 Hz；4 W、200 mJ、20 Hz)；联合组(n=48)采用铒铬铱钪镓石榴石激光照射磷酸酸蚀后的牙面，又分4个亚组，分别为1，2，3，4 W功率组(1 W、50 mJ、20 Hz；2 W、100 mJ、20 Hz；3 W、150 mJ、20 Hz；4 W、200 mJ、20 Hz)。每组随机选2个样本进行扫描电子显微镜观察，将剩余样本与复合树脂进行粘接，进行剪切粘接强度测试。

结果与结论：①空白对照组剪切粘接强度最低，为(4.03±1.80) MPa；激光2 W功率组剪切粘接强度最高，为(14.12±3.23) MPa；其次为磷酸酸蚀组，为(13.11±3.23) MPa；②扫描电子显微镜显示，空白对照组牙本质表面满布碎屑，玷污层覆盖；磷酸酸蚀组牙本质小管口完全开放，管径增大，玷污层清除，表面平坦，可见少量碎屑，管间牙本质与管周牙本质无明显界限；激光组牙本质表面高低不平，呈鱼鳞片状，牙本质小管口开放，清晰可见，管周牙本质突出于管间牙本质呈“袖口状”，表面无玷污层，随着激光照射能量的提高，牙本质小管出现管栓，表面出现一些裂隙和断裂；联合组牙本质表面较激光组起伏更明显，整体脱矿程度比激光组更高，牙本质小管内栓塞、闭合现象更显著；③结果表明对于乳牙牙本质，最佳的铒铬铱钪镓石榴石酸蚀参数是2 W、20 Hz、100 mJ。

ORCID: 0000-0001-9547-9795(史剑杰)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 口腔生物材料, Er,Cr:YSGG激光, 乳牙牙本质, 剪切粘接强度, 粘接界面形态, 生物材料

Abstract:

BACKGROUND: Pretreatment of dental hard tissues is critical for a success in tooth bonding repair.

OBJECTIVE: To explore the shear bond strength of nano-hybrid resin bonded to the primary tooth dentin after conditioning by 35% phosphoric acid, erbium, chromium:yttrium scandium gallium garnet (Er,Cr:YSGG) irradiation or their combined use.
METHODS: 120 extracted primary incisors with enamel removal were randomly divided into three groups and dealt with the following pretreatments: in control group (n=12), the tooth surface was without any processing; in acid etching group (n=12), the tooth surface was etched using 35% phosphoric acid; in laser group (n=48), the tooth surface was treated by Er,Cr:YSGG laser at four power outputs (1 W,   50 mJ, 20 Hz; 2 W, 100 mJ, 20 Hz; 3 W, 150 mJ, 20 Hz; 4 W, 200 mJ, 20 Hz); in combined group (n=48), the tooth surface was treated with 35% phosphoric acid followed by Er,Cr:YSGG laser irradiation at four power outputs (1 W, 50 mJ, 20 Hz; 2 W, 100 mJ, 20 Hz; 3 W, 150 mJ,  20 Hz; 4 W, 200 mJ, 20 Hz). Two samples from each group were randomly selected and observed using scanning electron microscope, and the residual samples were bonded with the composite resin for shear bonding strength test.

RESULTS AND CONCLUSION: (1) The shear bond strength of the dentine was lowest in the control group [(4.03±1.80) MPa], and highest in the 2 W laser group [(14.12±3.23) MPa], followed by the acid etching group [(13.11±3.23) MPa]. (2) Under the scanning electron microscope, the dentin surface of the control group was covered with a smear layer and debris. Dentin tubule of the acid etching group completely opened, with increased pipe diameter, the smear layer was removed presenting with the smooth surface on which only a little debris was seen, and no marked boundary existed between the pertitubular dentin and intertubular dentin. The dentin surface in the laser group was rugged and presented with fish scale-shaped flakes, the dentin tubule opened, and the intertubular dentin seemed to ablate more than the pertitubular dentin, showing a cuff-like protrusion of the dentinal tubules without smear layer. As the laser energy improved, blocking of the dentin tubule occurred, and some cracks and fractures appeared on the dentin surface. Fluctuant dentin surface was detected in the combined group, in which overall demineralization was higher and embolism and clogging of the dentin tubule was more prominent compared with the laser group. Overall, Er,Cr:YSGG laser ablation of the primary teeth, prior to the adhesive protocol, influences the shear bond strength to the primary tooth dentin and the best parameters for Er,Cr:YSGG laser are 2 W, 20 Hz, 100 mJ.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Dental Prosthesis, Lasers, Shear Strength, Tissue Engineering

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